Journal: Children

Article Title: Perspective on Similarities and Possible Overlaps of Congenital Disease Formation—Exemplified on a Case of Congenital Diaphragmatic Hernia and Neuroblastoma in a Neonate

doi: 10.3390/children8020163

Figure Lengend Snippet: H&E (Hematoxylin and Eosin) and immunohistochemical staining of primary tumor and metastases. ( A ) H&E staining of primary tumor shows histomorphological characteristics of poorly differentiated, Schwannian stroma poor neuroblastoma; ( B ) aquaporin 1 (AQP1) immunohistochemical staining and hematoxylin counterstaining of the liver metastases reveals dissolution of the periportal structure in the tumor-infiltrated regions. AQP1 positivity is observed in metastatic areas as well as in vascular endothelium (which can serve as an internal positive control). ( C ) AQP1, CXCR4 and MAP2 (microtubule associated protein) immunofluorescence staining of the primary tumor. Nuclei staining with DAPI (4′,6-diamidin-2-phenylindol). Next to AQP1, which is expressed in vascular structures as well as specific tumor areas, there is some expression of CXCR4 as a marker for metastatic homing as well as MAP2 expression in some areas indicating a more mature neuronal phenotype. Overall, tumor and metastases express experimental features that can be associated with a more aggressive phenotype.

Article Snippet: Immunohistochemistry staining was performed using the HRP-AEC-System from R&D Systems (Biotechnology Company, Minneapolis, MN, USA) with polyclonal rabbit anti-AQP1 antibody (Merck Millipore, Darmstast, Germany) at a dilution of 1:400 and counterstaining was performed with Mayer’s hematoxylin solution (Spitalpharmazie, Basel, Switzerland).

Techniques: Immunohistochemical staining, Staining, Positive Control, Immunofluorescence, Expressing, Marker